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. 2020 Jun 30;18(6):e3000732. doi: 10.1371/journal.pbio.3000732

Fig 7. Clinical relevance of the ROS/AMPK/EP300/β-catenin axis in human colorectal cancer.

Fig 7

(A) Histochemical staining with PAS preceded or not by digestion of consecutive sections with diastase (PAS/D) to reveal glycogen content (purple). Representative pictures with longitudinal (top) and transversal (bottom) sections. Scale bars: 50 μm. (B) Glycogen stores in human healthy colon cells HIEC 6 cultured for 24 h with low (1.5 mM) or high (25 mM) glucose revealed by PAS and PAS/D staining; scale bars: 25 μm. (C) Representative photographs of glycogen content stained with PAS in relation with ROS (8-OHdG), pAMPK (T172), pEP300 (S89), and β-catenin by immunohistochemical analysis in tumor versus healthy human bowel sections. Crypts are highlighted with yellow dashed line. Scale bars: 50 μm. (D) TMA analysis with 95 cores (0.6 mm diameter) of preselected regions of untreated CRC, stage 2. See Materials and Methods section and S1 Table for clinicopathological characteristics. Positive correlations were found between the nodes of the ROS/AMPK/EP300/β-catenin axis with correlation coefficients and P values shown. 8-OHdG, 8-hydroxy-2'-deoxyguanosine; CRC, colorectal cancer; pAMPKα (T172) phospho-AMP-activated protein kinase alpha (Threonine 172); PAS, Periodic Acid-Schiff; PAS/D, Periodic Acid-Schiff with alpha amylase; pEP300 (S89), phospho-Histone acetyltransferase p300 (Serine 89); ROS, reactive oxygen species; TMA, tissue microarray.