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. 2020 Jun 26;12:5131–5139. doi: 10.2147/CMAR.S244748

Figure 1.

Figure 1

ACT001 inhibited the growth of murine triple-negative breast cancer (TNBC) cell line 4T1 in vitro and in vivo. (A) Molecular structure of ACT001. (B) Murine 4T1 cells were treated with ACT001 at 20, 40, 60, and 80 μM for 72 h, and cell viability was measured by CCK-8 assay. (C and D) 4T1 tumor-bearing mice were treated with sterile water or 100 mg/kg ACT001 for 18 d. 4T1 tumor volumes and photographs of 4T1 tumors are shown. (E) Apoptotic cells in 4T1 tumors were visualized using TUNEL staining. (F) Survival curves of 4T1 tumor-bearing mice (n = 8). (G) BAX and β-actin protein expression in 4T1 cells after 60 μM ACT001 treatment and in 4T1 tumors after 100 mg/kg ACT001 treatment as determined by Western blotting. Data are shown as means ± SD values for each treatment group (n = 5). *P < 0.05 versus vehicle control.