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. 2020 Jun 23;31(12):107806. doi: 10.1016/j.celrep.2020.107806

Figure 5.

Figure 5

mTOR Inhibition Decreases Sapienate Metabolism In Vivo

(A) Relative FADS2 mRNA expression in HUH7 orthotopic liver xenografts treated with vehicle (n = 15) or Torin1 (20 mg/kg daily, i.p., for 3 days; n = 7) normalized to control tumor. Treatment was started 10 days after tumor initiation. The mice were sacrificed 2 h after the last dose of Torin1. Bar graphs are presented as mean ± SD from biological independent samples. Statistical testing was performed by a two-sided unpaired Student’s t test.

(B) Sapienate to palmitate ratio in HUH7 orthotopic liver xenografts treated with vehicle (n = 24) or Torin1 (20 mg/kg daily, i.p., for 3 days; n = 6) normalized to control tumor. Treatment was started 10 days after tumor initiation. The mice were sacrificed 2 h after the last dose of Torin1. Bar graphs are presented as mean ± SD from biological independent samples. Statistical testing was performed by a two-sided unpaired Student’s t test.