Figure 4.

T-type Ca²⁺ channels, but not mGluRs, are required for PF. A, Representative recordings of PF from two different TRN neurons, in control conditions and in the presence of the mGluR1 antagonist JNJ 16259685 (1 μm) and the mGluR5 antagonist MTEP (10 μm). B, Summary data quantifying duration of evoked membrane depolarization in control (n = 111) and in the presence of mGluR 1/5 antagonists (n = 23). C, Representative recording from neuron with PF, initially held at a holding potential of −60 mV and then at −75 mV. D, Summary data quantifying evoked depolarization duration as a function of holding potential (n = 15 TRN neurons). E, Representative recordings in the presence of apamin and in the presence of apamin and the T-type Ca²⁺ channel antagonist TTA-P2 (1 μm). F, Summary data quantifying evoked depolarization duration (n = 50 in apamin, n = 17 in apamin + TTA-P2).