Figure 4. SOS1 was a target of miR-217. A, The relationship between miR-217 and SOS1 was predicted by TargetScan software. B, The luciferase activity of the reporter with SOS1 wild type (WT) or mutated (MUT) was evaluated by the luciferase reporter assay. C, After the transfection of miR-217 inhibitor, the expression of miR-217 in HL-60 and Kasumi-1 cells was measured by qRT-PCR. D, After acute myeloid leukemia (AML) cells were transfected with miR-217 mimic, control mimic, miR-217 inhibitor, or negative control (NC) inhibitor, the protein expression of SOS1 was determined by western blot. E, The expression of SOS1 in bone marrow tissues from AML patients and healthy donors was calculated by qRT-PCR. F, The correlation among SOS1, miR-217, and LINC01268 was evaluated by the Pearson correlation analysis. Data are reported as means±SD. **P<0.01 vs the control group (ANOVA and t-test).