Figure 5.

Identification of Hippocampal Transcripts Differentially Expressed According to Sex and Genotype

(A) RNA sequencing analysis was performed on Ctrl^Male(C-M), Atrx-cKO^Male(K-M), Ctrl^Fem(C-F), and Atrx-cKO^Fem(K-F) hippocampi (n = 3 each genotype), and an expression heatmap of the transcripts altered upon loss of ATRX was generated in male (left) and in female (right) mice, respectively (FDR < 0.1).

(B) Functional classification of sexually dimorphic transcripts identified in the hippocampus (biological processes, FDR < 0.05).

(C) List of genes included in the top category “synapse organization” identified by pathway analysis.

(D) Transcripts differentially expressed as a function of sex and genotype clustered into 4 categories. The number of genes in each cluster is indicated at right.

(E) Summary heatmaps of transcripts in clusters 1–4 identified in (D).

See also Figure S6 and Tables S1, S2, and S3.