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. 2020 Jun 18;9:e59264. doi: 10.7554/eLife.59264

Figure 6. Specific features of the LSU.

The LSU central protuberance lacks 5S rRNA, and the L7/L12 stalk consists of only two dimers. Superposition of E. coli 5S rRNA reveals no substantial protein replacement, apart from minor elements shown in red. The model of the native L7/L12 stalk reveals unusual conformation due to the presence of rRNA expansion H42-ES1 and mL104.

Figure 6.

Figure 6—figure supplement 1. Structure of the L7/L12 stalk.

Figure 6—figure supplement 1.

(A) Helical repeat protein mL104 bound to H42-ES1 forms a stabilizing interface for the proximal bL12m dimer, which results in a more rigid structure. (B) Comparison of uL10 between E. coli ribosome and T. thermophila mitoribosome shows that the linker domain α8 is straight and rigid, lacking the representative kinks from bacteria.