Skip to main content
. Author manuscript; available in PMC: 2020 Sep 7.
Published in final edited form as: J Proteome Res. 2020 Jan 7;19(2):984–990. doi: 10.1021/acs.jproteome.9b00696

Figure 2. Solid-phase extraction with triethylammonium acetate promotes retention of ADP-ribosylated peptides on solid phase.

Figure 2.

(A) Urea-PAGE analyses of 32P-(ADPr)n-PARP1 digested with trypsin before (Input), and after solid-phase extraction on a C18 cartridge using trifluoroacetic acid (TFA) or triethylammonium acetate (TEAA). Flowthrough (FT), wash (W), and elution (E) fractions are included in separate lanes. Gel was analyzed by autoradiography. (B) Relative recovery of radioactive species in (A). Radioactive counts were acquired by scintillation counting, and the relative recovery was calculated as a fraction of the input (n=3).