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. 2020 Jun 25;13:263–274. doi: 10.2147/JIR.S252433

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© 2020 Sae-khow et al.

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Effect of ascorbate on NET formation. Representative fluorescence images (400X) of isolated neutrophils from patients with sepsis and healthy controls treated with 1, 10 and 40 mM of ascorbate for 2h and stained with DAPI staining for NET identification (A). NETs counted per 100 neutrophils (n=20 for both groups) (B), the levels of dsDNA (C) and PADI4 mRNA expression (D) from patients with sepsis and healthy controls (n=10 for both groups) treated with different concentrations of ascorbate (1, 5, 10, 20 and 40 mM) for 2h.

Notes: ****p<0.0001, ***p<0.001, **p<0.01. ^##p<0.01, ^####p<0.001; when compared between groups.