Figure 2.

Hepatocyte AAV-GFP Expression Varies between Human Donors

(A) FNRG mice humanized with 8 different PHH donors were challenged with AAVLK03-GFP, and hepatocyte GFP expression was quantified by flow cytometry. Symbols represent individual mice; black bar represents the mean of the group. The p values were calculated by Mann-Whitney test and depicted when <0.05. (B) Peak mean hAlb serum levels of groups of FNRG mice transplanted with various PHH donors was plotted against mean percentage of GFP⁺ human hepatocytes. Symbols represent means of groups; correlation was calculated by Pearson test; colors correspond to those in (A). (C) FNRG mice humanized with 5 PHH donors were challenged with AAVSpark100-GFP, and hepatocyte GFP expression was quantified by flow cytometry. Symbols represent individual mice. (D) Human hepatocyte GFP⁺ percentages were compared between AAVLK03-GFP and AAVSpark100-GFP challenged mice humanized with 5 PHH donors. Symbols represent mean percentages of groups; correlation was calculated by Pearson test; colors correspond to those in (A) and (C).