Effects of Ile, Leu and Val additions on bacitracin production. A: Effects of 40 mg/L Ile, Leu and Val addition at different time points on bacitracin production, respectively. B: Effects of different concentrations of Ile and Leu additions at 18 h on bacitracin production, respectively. Each experiment was repeated at least three times, and data are represented as the means of three replicates and bars represent the standard deviations, ∗, P < 0.05; and ∗∗, P < 0.01 indicate the significance levels between recombinant strains and control strain.