Table 1.
The strains and plasmids used in this research.
| Strains and plasmids | Relevant properties | Source of reference |
|---|---|---|
| Strains | ||
| Escherichia coli DH5α | supE44 ΔlacU169 (f 80 lacZΔM15) hsd R17 recA1 gyrA96 thi1 relA1 | This study |
| Bacillus licheniformis DW2 | Wide-type CCTCC M2011344 | CCTCC |
| DW2/pHY-ilvA | DW2 harboring IlvA expression vector pHY-ilvA | This study |
| DW2/pHY-ilvBN | DW2 harboring IlvBN expression vector pHY-ilvBN | This study |
| DW2/pHY-ilvCD | DW2 harboring IlvCD expression vector pHY-ilvCD | This study |
| DW2/pHY-leuA | DW2 harboring LeuA expression vector pHY-leuA | This study |
| DW2/pHY-leuB | DW2 harboring LeuB expression vector pHY-leuB | This study |
| DW2/pHY-leuC | DW2 harboring LeuC expression vector pHY-leuC | This study |
| DW2/pHY-leuD | DW2 harboring LeuD expression vector pHY-leuD | This study |
| DW2/pHY-300 | DW2 harboring pHY300PLK, as control strain | This study |
| DW2/pHY-ilvBNfbr | DW2 harboring IlvBNfbr expression vector pHY-ilvBNfbr | This study |
| DW2/pHY-leuAfbr | DW2 harboring LeuAfbr expression vector pHY-leuAfbr | This study |
| DW2-ilvBNfbr | IlvBNfbr integration overexpression strain | This study |
| DW2-leuAfbr | LeuAfbr integration overexpression strain | This study |
| DW-BCAA1 | IlvBNfbr and LeuAfbr integration overexpression strain | This study |
| DW-BCAA1/pHY300 | DW-BCAA1 harboring pHY300PLK | This study |
| DW-BCAA1/pHY-IlvE | DW-BCAA1 harboring IlvE expression vector pHY-IlvE | This study |
| DW-BCAA1/pHY-YbgE | DW-BCAA1 harboring YbgE expression vector pHY-YbgE | This study |
| DW-BCAA1/pHY-YwaA | DW-BCAA1 harboring YwaA expression vector pHY-YwaA | This study |
| DW-BCAA2 | Overexpression of YbgE via promoter replacement in DW-BCAA1 | This study |
| DW2△yvbW | Deleting yvbW in DW2 | This study |
| DW2/pHY-YvbW | DW2 harboring YvbW expression vector pHY-YvbW | This study |
| DW2△BraB | Deleting braB in DW2 | This study |
| DW2/pHY-BraB | DW2 harboring BraB expression vector pHY-BraB | This study |
| DW-BCAA3 | Overexpression of YvbW via promoter replacement in DW-BCAA2 | This study |
| DW-BCAA4 | Overexpression of BraB via promoter replacement in DW-BCAA2 | This study |
| DW-BCAA5 | Simultaneously overexpressing YvbW and BraB via promoter replacement in DW-BCAA2 | This study |
| DW-BCAA6 | Deleting regulator gene lrpC in DW-BCAA5 | This study |
| Plasmids | ||
| pHY300PLK | E. coli and B. s shuttle vector; Ampr, Tetr | Lab collection |
| T2(2)-Ori | Bacillus knockout vector; Kanr | Lab collection |
| pHY-ilvA | IlvA expression vcctor based on pHY300PLK | This study |
| pHY-ilvBN | IlvBN expression vcctor based on pHY300PLK | This study |
| pHY-ilvCD | IlvCD expression vcctor based on pHY300PLK | This study |
| pHY-leuA | LeuA expression vcctor based on pHY300PLK | This study |
| pHY-leuB | LeuB expression vcctor based on pHY300PLK | This study |
| pHY-leuC | LeuC expression vcctor based on pHY300PLK | This study |
| pHY-leuD | LeuD expression vcctor based on pHY300PLK | This study |
| pHY-ilvBNfbr | IlvBNfbr expression vcctor based on pHY300PLK | This study |
| pHY-leuAfbr | LeuAfbr expression vcctor based on pHY300PLK | This study |
| pHY-IlvE | IlvE expression vcctor based on pHY300PLK | This study |
| pHY-YbgE | YbgE expression vcctor based on pHY300PLK | This study |
| pHY-YwaA | YwaA expression vcctor based on pHY300PLK | This study |
| T2-::ilvBNfbr | T2(2)-Ori-ilvBNfbr(A + tnrA + B); to overexpress ilvBNfbr | This study |
| T2-::leuAfbr | T2(2)-Ori-leuAfbr(A + tnrA + B); to overexpress leuAfbr | This study |
| T2-PbacA-PybgE | T2(2)-Ori-PybgE(A + PbacA + B); to replace the promoter of ybgE by PbacA | This study |
| T2-PbacA-PyvbW | T2(2)-Ori-PyvbW(A + PbacA + B); to replace the promoter of yvbW by PbacA | This study |
| T2-PbacA-PbraB | T2(2)-Ori-PbraB(A + PbacA + B); to replace the promoter of braB by PbacA | This study |
| T2-lrpC | T2(2)-Ori-lrpC(A + B); to delete lrpC | This study |