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. 2020 Jun 24;11:1300. doi: 10.3389/fimmu.2020.01300

Figure 3.

Figure 3

SHH agonist promotes FLSs proliferation via JNK. (A) FLSs viability was assessed by Cell Counting Kit-8 assay after FLSs were treated with SHH agonist (SAG, 1 μM) alone or SAG with JNK inhibitor (SP600125, 20 μM) for 48 h (n = 4). (B–G) Cell cycle analysis, Ki-67 expression and cell apoptosis were assessed by flow cytometry. For the detection of cell cycle distribution (B,C) and expression of Ki-67 (D,E), FLSs were treated with SAG (1 μM) with or without SP600125 (20 μM) for 18 h. For the detection of cell apoptosis (F,G), FLSs were treated with SAG (1 μM) with or without SP600125 (20 μM) for 24 h. Percentage of apoptosis was defined by the number of AnnexinV+PI− cells. FLSs in control group were treated with vehicle (DMSO supplemented in DMEM with 10%FBS). The results are shown as the mean ± S.D. Data were analyzed using ANOVA and post hoc comparisons were made by Dunnett's test (A,B,D,F). *P < 0.05 vs. control group. **P < 0.01 vs. control group. ***P < 0.001 vs. control group. ns, non-significant; CON, control group; SP, SP600125.