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. 2020 Jun 24;11:1172. doi: 10.3389/fmicb.2020.01172

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Copyright © 2020 Santajit, Seesuay, Mahasongkram, Sookrung, Pumirat, Ampawong, Reamtong, Chongsa-Nguan, Chaicumpa and Indrawattana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HuscFvs rescue HeLa cells from 3O-C12-HSL-mediated apoptosis. (A) The percentages of apoptotic cells of different treatment conditions, i.e., HeLa cells in medium alone, cells exposed to 50 μM 3O-C12-HSL (C12), cells added with 3O-C12-HSL mixed with various amounts of HuscFvs. (B) Density plots of flow cytometric analysis of doubly stained HeLa cells as in (A) (representative of one of the three reproducible experiments). The percent apoptotic cells caused by the 3O-C12-HSL was reduced significantly in the presence of HuscFvs.