FIGURE 4.

Co-expression of LPMO and LMW hydroquinone pathways at an early brown rot stage in G. trabeum. (A) The hydroquinone chelator synthesis pathway and the enzyme homologs catalyzing each step are proposed based on standard pathways. Genes that were significantly upregulated (*, FDR < 0.05 by RNA-seq) in early brown rot basically covered all the required key enzyme components of the pathway, although several OMT, 3HB6H and SAH genes were clearly upregulated at late decay stages (x, FDR < 0.05) (B). Expression timing of G. trabeum LPMO genes showed that AA9a was significantly upregulated in early brown rot stages, and these were co-expressed with genes for metabolites produced in proposed pathway for hydroquinone synthesis. (C) Expression levels for GtrAA9 genes during early-, mid- and late-decay stages.