TABLE 3.

Summary of alpha-diversity measurements of the rumen microbiota.

Diversity measurements	Donor†	Treatment				SEM	P-values
		Ctrl*		Inoc*
		Ctrl-A	Ctrl-B	Inoc-A	Inoc-B		Trt♣	Donor†
Observed ASVs	370	106	77	104	117	9.43	0.283	0.690
Chao1	377	125	92	122	140	12.63	0.353	0.652
Evenness	0.86	0.58	0.51	0.48	0.54	0.02	0.446	0.367
Faith’s phylogenetic diversity	23.23	9.10	5.79	7.92	9.39	0.64	0.288	0.429
Shannon index	7.30	3.84	3.18	3.24	3.62	0.14	0.884	0.375
Simpson index	0.98	0.81	0.75	0.74	0.79	0.02	0.869	0.474

*Ctrl, control calves receiving autoclaved rumen fluid; Inoc, calves receiving fresh rumen fluid. standard error of mean. calculated using F-test. ^♣ treatment effect: inoculated with autoclaved rumen fluid (Ctrl) or fresh rumen fluid (Inoc). calculated based on the two groups of Inoc calves (Inoc-A and Inoc-B). ^† Each group of calves received rumen fluid from only one of four donor cows throughout the experiment. Only the sequence data of the donors for the included calves were presented. Good’s coverage exceeded 99% for all the samples (data not shown).