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. 2020 Jun 24;11:1403. doi: 10.3389/fmicb.2020.01403

TABLE 4.

Number of detected functional features of the rumen microbiome using by PICRUSt2.

Functional databases Donor Treatment
SEMInline graphic P-valuesInline graphic
Ctrl*
Inoc*

Ctrl-A Ctrl-B Inoc-A Inoc-B Trt DonorInline graphic
KO 3,450 3,454 3,133 3,699 3,527 116.04 0.152 0.655
COG 3,116 3,080 2,801 3,217 3,077 74.07 0.138 0.528
EC 1,248 1,224 1,126 1,281 1,248 29.89 0.113 0.725
PFAM 4,514 4,322 3,944 4,528 4,398 111.89 0.120 0.707
KEGG-modules 229 223 208 227 223 3.66 0.155 0.760
KEGG-pathways 125 117 111 121 119 1.70 0.066 0.689
MetaCyc-pathways 260 260 238 268 261 6.04 0.177 0.707

* Ctrl, control calves receiving autoclaved rumen fluid; Inoc, calves receiving fresh rumen fluid. Inline graphic standard error of mean. Inline graphic calculated using F-test. Treatment effect: inoculated with autoclaved rumen fluid (Ctrl) or fresh rumen fluid (Inoc). Inline graphic calculated based on the two groups of Inoc calves (Inoc-A and Inoc-B). Each group of calves received rumen fluid from only one of four donor cows throughout the experiment. Only the sequence data of the donors for the included calves were presented.