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. 2020 Jun 24;11:1358. doi: 10.3389/fmicb.2020.01358

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Copyright © 2020 Peters, McCutcheon and Dennis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DLP3 requires a functional type IV pilus to infect host strains D1585 and 280. Phage DLP3 is capable of infecting wildtype S. maltophilia strains D1585 and 280, infecting both at 10⁹ PFU/mL, whereas phage infection is blocked in the ΔpilA and ΔpilT mutants. Complementation with the endogenous genes restores phage infection to wildtype levels. DLP3 plaquing ability on strain D1571 is shown for titer calculation, however, no mutants were constructed in this background. Images are representative of three biological replicates, each with three technical replicates.