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. 2020 Jun 30;10:10677. doi: 10.1038/s41598-020-67689-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of 0.5% γ-oryzanol (GORZ) treatment on chronic 10% ethanol-induced behavioral changes in the elevated plus maze (EPM) test (Experiment 2). The EPM test was conducted on W4 from ZT 5 to 7 with an ethanol wash out to measure anxiety-related behaviors. The (A) Distance travelled in the open arm (m), (B) Time in the open arm (s), (C) Number of entries into the open arm, (D) Distance travelled in the closed arm (m), (E) Time in the closed arm (s), and (F) Number of entries into the closed arm were measured. All values are expressed as mean ± standard error (SE); n = 10–12 mice per group. Two-way analysis of variance (ANOVA) with Tukey test *P < 0.05, **P < 0.01, ***P < 0.001, and Kruskal–Wallis tests followed by Dunn’s multiple comparisons test ^$P < 0.05, ^$$$P < 0.001 were used to determine significance.