Fig. 8. Total anthocyanin content and expression patterns of related ABGs in different transgenic Arabidopsis lines.

a–e Thirty-day-old Arabidopsis seedlings. a WT Arabidopsis; b T₃ CaMV35S:cBrMYB2 lines; c T₃ CaMV35S:gBrMYB2 lines; d T₃ CaMV35S:cBrmyb2 lines; e T₃ CaMV35S:gBrmyb2 lines. f Semi-qRT-PCR analysis of full-length BrMYB2. g Total anthocyanin contents of samples. h qRT-PCR analysis of BrMYB2, and Line 01 of the T₃gBrmyb2 lines served as the control. i–r Expression patterns of ABGs in Arabidopsis, and the WT line served as the control. The values are presented as the means ± SDs (n = 3). The different letters above each column are significantly different at p < 0.05 according to Duncan’s test. The first ‘c’ and ‘g’ letters of the abscissa labels represent cDNA and gDNA, respectively; the second letters ‘M’ and ‘m’ represent BrMYB2 and Brmyb2, respectively