FIGURE 3.

Hyper-phosphorylated tau-containing cells and threads following unilateral hippocampal injection of sarkosyl-insoluble fractions from AGD in WT mice inoculated at 7 months and killed at the age of 10 months (3 months of survival) (A,B); inoculated at the age of 3 months and killed at the age of 6 months (3 months survival) (C); inoculated at the age of 12 months and killed at the age of 19 months (7 months survival) (D–F); and inoculated at the age of 3 months and killed at the age of 10 months (survival 7 months) (E–H). Neurons in the ipsilateral hippocampus contain granular hyper-phosphorylated tau deposits as revealed with the AT8 antibody without morphological features of tangles (A); these deposits are stained with antibodies against 4Rtau and 3Rtau (G,H). Grains (dots) in CA1 region of the hippocampus, dentate gyrus, fimbria, and corpus callosum are recognized with the antibody AT8 (B,C,E,F), anti P-tau Ser262 (D), and antibodies against 4Rtau and 3Rtau (G–I) (thin arrows). Threads and coiled bodies containing hyper-phosphorylated tau are seen in the ipsilateral and contralateral corpus callosum in mice surviving 7 months after inoculation. Paraffin sections slightly counterstained with hematoxylin. CA1, region of the hippocampus; DG, dentate gyrus; fimbr, fimbria; ipsi contr CC, ipsi- and contralateral corpus callosum; (A–F), bar = 25 μm; (G–I), bar = 25 μm. Grains, coiled bodies and grains, insets in (A,E,H), respectively, bar = 7.5 μm.