FIGURE 7.

(A–D) WT mice unilaterally inoculated in the hippocampus with sarkosyl-insoluble fractions from AGD at the age of 12 months and killed at 19 months (7 months survival) showing tau-immunofluorescent CA1 neurons (asterisks) in the ipsilateral hippocampus (A), and deposits of hyper-phosphorylated tau in the cytoplasm of oligodendrocytes (B,C; arrows) as revealed with double-labeling immunofluorescence to NeuN (green) and P-tauThr181 (red), and Olig2 (green) and AT8 (red), respectively. Double-labeling immunofluorescence to phosphorylated p38 (p38-P, p38-PThr180-182) (green) and AT8 (red) in WT mice inoculated with sarkosyl-insoluble fractions from AGD at the age of 12 months and killed at the age of 19 months (7 months survival) (D). Active p38 kinase (p38-P) co-localizes with tau deposits in tau-positive grains (thick arrows) and threads in the hippocampus. E: WT mouse unilaterally inoculated in the hippocampus with sarkosyl-insoluble fractions from PART at the age of 12 months and killed at 19 months (7 months survival) showing tau-immunofluorescent oligodendrocyte (thin arrow) and threads (short arrows) in the contralateral corpus callosum. Paraffin sections, nuclei stained with DRAQ5^TM (blue); (A), bar = 10 μm; (B), bar = 30 μm; (C,D), bar = 10 μm; (E) = 25 μm.