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. 2020 Jun 30;10:10597. doi: 10.1038/s41598-020-67325-7

Figure 4.

Figure 4

Acquisition of stemness in vitro and in vivo. (A) Sphere forming potentials of all lung cells following TGF-β treatment were higher than those of cells untreated with TGF-β. (B, C) Tumor formation abilities in BEAS-2B, A549, and H226 cells treated with or without TGF-β were compared following injection into BALB/c nude mice. Numbers of mice that developed tumors were higher in TGF-β treated cell groups compared to those in TGF-β untreated cell groups. Weight and size of the tumors were enhanced following injection of BEAS-2B (1 × 106 and 5 × 106), A549 (5 × 106), and H226 (5 × 106) cells treated with TGF-β compared with those in TGF-β untreated cell groups. Data are presented as mean ± SE (*P < 0.05, P < 0.01).