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. 2020 Jun 30;10:10597. doi: 10.1038/s41598-020-67325-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative demonstration of CpG site methylation changes of CD87 promoter region in BEAS-2B and A549 cells. Bisulfite sequencing showed six CpG sites presented in underlined letters within a 100-bp promoter region of CD87 gene. Demethylated CpG sites following TGF-β treatment are indicated by red letters. Heterozygote C/T double peaks are indicated by Y.