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. 2020 Jun 30;11(6):493. doi: 10.1038/s41419-020-2687-6

Fig. 4. β-catenin regulates eNOS under flow.

Fig. 4

a Cell lysates were obtained from HUVEC exposed to UF or DF for 72 h (n = 4). b EC transfected with non-targeting scrambled or β-catenin targeting siRNA (100 nM) were exposed to orbital flow for 72 h (n = 5). c Cell lysates obtained from HUVEC exposed to UF or DF for 72 h and treated with vehicle or LiCl (20 mM) for the last 4 h (n = 3). a–c Cell lysates were analysed by western blot with the indicated antibodies. Results are expressed as the densitometric ratio of phospho-eNOS/eNOS to total eNOS/calnexin and shown relative to levels in UF in vehicle or scr treated conditions; analysis by paired Student’s t test (a) or analysis by one-way ANOVA with repeated measures (b, c), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.