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. 2020 Jun 30;9:110. doi: 10.1038/s41377-020-00348-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Imaging comparison of a uniform fluorescein layer placed 150 μm away from a thin (≲50 µm) scattering layer: (a) conventional 2P microscopy (b), pupil F-SHARP imaging (c) and conjugate F-SHARP (d). Intensity profile of the fluorescence image captured with the three configurations along the dotted line of b–d (e). Images in b through d were acquired with the same laser power and pixel dwell time and normalized to the maximum value of the 3.