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. 2020 Jun 30;11:3279. doi: 10.1038/s41467-020-17047-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative native EGFP (green) expression following rAAV2-CB6-PI-EGFP or rAAVv66-CB6-PI-EGFP injection via unilateral intrahippocampal administration. Scale bars = 700 μm. b Quantification of EGFP-positive surface normalized to DAPI-positive surface from panel a data. Data are presented as the mean ± SD; n = 3 animals/group from one experiment. ****P < 0.0001, two-tailed Student’s t test. c Coronal brain schematic depicting sub-anatomical regions of interest in both contralateral and ipsilateral hemispheres (adapted from Allen Mouse Brain Atlas). Cornu ammonis (CA1–CA4), dentate gyrus (DG), corpus callosum (CC), and cortex (CTX). d Representative high-magnification images of rAAVv66 transduced sub-anatomical regions from n = 3 animals from one experiment. Scale bars = 50 μm.