Table 1.
Primer pairs for site directed mutagenesis to generate point mutations in the AAVv66 capsid ORF.
| Mutation in AAVv66 | Forward primer (5′-3′) (lowercase = mutated bases) | Reverse primer (5′-3′) |
|---|---|---|
| Q39K | AGAGCGGCATaagGACGACAGCA | GCGGGCTTTGGTGGTGGT |
| A151V | GCATTCTCCTgtgGAGCCAGACT | TCTACCGGCCTCTTTTTTCC |
| K447R | TTACTTGAGCagaACAAACGCTC | TACAGATACTGGTCGATC |
| A450T | CAAAACAAACactCCAAGCGGAAC | CTCAAGTAATACAGATACTGG |
| M457Q | AACCACCACGcagTCCAGGCTTC | CCGCTTGGAGCGTTTGTT |
| A492S | ATCAAAAACAtctGCGGATAACAACAACAGTG | ACTCGCTGCTGGCGGTAA |
| D499E | CAACAACAGTgaaTATTCGTGGAC | TTATCCGCAGCTGTTTTTG |
| Y533F | TGAAGAAAAAtttTTTCCTCAGAGCGGGGTTC | TCGTCCTTGTGGCTGGCC |
| D546G | TGGAAAACAAggcTCGGGAAAAA | AAGATGAGAACCCCGCTC |
| G548E | ACAAGACTCGgagAAAACTAATGTG | TTTCCAAAGATGAGAACC |
| S585R | CAACCTCCAGagaGGCAACACAC | GTAGATACAGAACCATACTGCTC |
| T588R | GAGCGGCAACagaCAGGCAGCCA | TGGAGGTTGGTAGATACAGAACCATACTG |
| T593A | GGCAGCCACCgcaGATGTCAACA | TGTGTGTTGCCGCTCTGG |