Table 1.
PGRs | Conc. (mg/l) | Explants | ||||
---|---|---|---|---|---|---|
Node | Leaf | |||||
Explants responding for shoot regeneration % | No. of shoots ± SE | Shoot length ± SE (cm) | Morphological response | |||
Control | 0.0 | 79.4 ± 0.44i | 1.3 ± 0.12j | 2.3 ± 0.12a | R, S | R, S |
BAP | 0.5 | 82.8 ± 0.40h | 6.0 ± 0.12b | 1.6 ± 0.12cde | S | N |
1 | 97.3 ± 0.46a | 7.8 ± 0.15a | 1.5 ± 0.09def | S | N | |
2 | 91.2 ± 0.60d | 4.5 ± 0.20c | 1.5 ± 0.15def | S* | N | |
3 | 88.2 ± 0.55fg | 3.3 ± 0.15e | 1.0 ± 0.15h | S* | * | |
Kin | 0.5 | 91.9 ± 0.44bcde | 1.6 ± 0.06hi | 1.9 ± 0.03b | S | * |
1 | 93.3 ± 0.65bcde | 1.6 ± 0.01hi | 1.8 ± 0.09bc | S | * | |
2 | 89.5 ± 0.87ef | 1.7 ± 0.01h | 1.7 ± 0.06cd | S | * | |
3 | 85.8 ± 1.47g | 1.6 ± 0.01hi | 1.3 ± 0.06fg | S* | * | |
TDZ | 0.5 | 77.2 ± 0.61i | 1.1 ± 0.03jk | 0.5 ± 0.06ij | S* | * |
1 | 77.2 ± 0.60i | 1.0 ± 0.06k | 0.4 ± 0.03ijk | S* | ** | |
2 | 74.2 ± 0.47j | 1.0 ± 0.00k | 0.3 ± 0.01ijk | S* | ** | |
3 | 71.1 ± 0.61k | 0.9 ± 0.00k | 0.2 ± 0.00k | S* | ** |
The value represents the means of three replicates with standard error (SE). Values sharing the different alphabets are statistically different from each other at P < 0.05.
R root, S shoot, N no change.
*Callus induction.
**Callus induction and proliferation.