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. 2020 Jun 30;10:10668. doi: 10.1038/s41598-020-67637-8

Table 1.

Effect of different concentrations of cytokinins on shoot regeneration in Phyllanthus tenellus Roxb.

PGRs Conc. (mg/l) Explants
Node Leaf
Explants responding for shoot regeneration % No. of shoots ± SE Shoot length ± SE (cm) Morphological response
Control 0.0 79.4 ± 0.44i 1.3 ± 0.12j 2.3 ± 0.12a R, S R, S
BAP 0.5 82.8 ± 0.40h 6.0 ± 0.12b 1.6 ± 0.12cde S N
1 97.3 ± 0.46a 7.8 ± 0.15a 1.5 ± 0.09def S N
2 91.2 ± 0.60d 4.5 ± 0.20c 1.5 ± 0.15def S* N
3 88.2 ± 0.55fg 3.3 ± 0.15e 1.0 ± 0.15h S* *
Kin 0.5 91.9 ± 0.44bcde 1.6 ± 0.06hi 1.9 ± 0.03b S *
1 93.3 ± 0.65bcde 1.6 ± 0.01hi 1.8 ± 0.09bc S *
2 89.5 ± 0.87ef 1.7 ± 0.01h 1.7 ± 0.06cd S *
3 85.8 ± 1.47g 1.6 ± 0.01hi 1.3 ± 0.06fg S* *
TDZ 0.5 77.2 ± 0.61i 1.1 ± 0.03jk 0.5 ± 0.06ij S* *
1 77.2 ± 0.60i 1.0 ± 0.06k 0.4 ± 0.03ijk S* **
2 74.2 ± 0.47j 1.0 ± 0.00k 0.3 ± 0.01ijk S* **
3 71.1 ± 0.61k 0.9 ± 0.00k 0.2 ± 0.00k S* **

The value represents the means of three replicates with standard error (SE). Values sharing the different alphabets are statistically different from each other at P < 0.05.

R root, S shoot, N no change.

*Callus induction.

**Callus induction and proliferation.