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. 2020 Jun 30;10:10668. doi: 10.1038/s41598-020-67637-8

Table 2.

Effect of different concentrations of NAA alone and in combination with cytokinins on growth of callus in Phyllanthus tenellus Roxb.

PGRs Conc. mg/l Explants
Node Leaf
Nature of callus FW (g) DW (mg) Nature of callus FW (g) DW (mg)
Control 00 N N N N N N
NAA 0.5 WGC 0.73 ± 0.6c 177.5 ± 3.1c GWC 1.16 ± 0.3c 187.2 ± 2.3c
1 WGC 1.12 ± 1.1b 201.4 ± 1.4b GWC 1.81 ± 0.8a 232.6 ± 2.7b
2 WGC 1.21 ± 1.2a 212.7 ± 3.3a GWC 1.88 ± 0.7b 237.2 ± 1.8a
3 WGC 1.22 ± 1.3a 204.8 ± 2.3b GWC 1.75 ± 0.3b 228.6 ± 1.9b
NAA + BAP 0.5 GC 1.20 ± 1.1a 205.2 ± 1.3b GC 1.25 ± 0.3b 225.3 ± 1.3a
1 GC 1.00 ± 0.6b 195.6 ± 0.3c GC 1.21 ± 0.6a 218.2 ± 1.1b
2 GC 0.75 ± 0.4c 181.8 ± 0.8d GC 0.86 ± 1.4c 199.5 ± 0.7c
3 GC 0.71 ± 0.3c 175.3 ± 0.7e GC 0.81 ± 1.7c 187.3 ± 1.2d
NAA + Kin 0.5 GWF 1.20 ± 0.3a 208.8 ± 5.5a GWF 1.81 ± 0.3c 229.4 ± 5.2a
1 GWF 0.95 ± 0.6b 189.8 ± 4.6b GWF 1.68 ± 0.8a 212.4 ± 2.8b
2 GWF 0.82 ± 0.8c 173.6 ± 4.1c GWF 1.42 ± 0.7b 201.6 ± 4.3c
3 GWF 0.76 ± 0.1d 162.5 ± 3.2d GWF 1.35 ± 0.3b 188.7 ± 2.3d
NAA + TDZ 0.5 GC 1.15 ± 0.2c 201.4 ± 1.1b GF 1.71 ± 0.2d 222.7 ± 5.6b
1 GC 1.12 ± 0.3a 183.3 ± 2.8a GF 1.62 ± 0.4c 204.6 ± 0.8a
2 GC 1.00 ± 0.4a 163.7 ± 1.4c GF 1.48 ± 1.2a 184.4 ± 4.1c
3 GC 0.92 ± 0.2b 152.3 ± 3.6d GF 1.23 ± 1.2b 161.3 ± 4.2c

The value represents the means of three replicates with standard error (SE). Values sharing the different alphabets are statistically different from each other at P < 0.05.

N no callus formation, WGC whitish green compact, GC green compact, GWF greenish white friable, GWC greenish white compact, GF green friable.