Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 30;10:10618. doi: 10.1038/s41598-020-67457-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The metabolic shift from OXPHOS to glycolysis was induced by genetic ablation of p32/C1QBP resulting in glucose addiction. (a) Wild type, p32^−/− and p32^−/− + p32 MEFs were subjected to Agilent Seahorse XF Cell Mito Stress Test to measure basal respiration, maximal respiration, proton leak and mitochondrial ATP production. Bar graphs illustrate the absolute value of OCR that was calculated from SI Fig S8a,b and plotted with SD as error bars. Student’s t test, *p < 0.05, ***p < 0.001. (b) Wild type, p32^−/− and p32^−/−+p32 MEFs were subjected to Agilent Seahorse XF glycolysis stress test to measure glycolysis, glycolytic capacity and glycolytic reserve. Bar graphs illustrate the absolute value of ECAR that was calculated from SI Fig S8c,d and plotted with SD as error bars. Student’s t test, **p < 0.01, ***p < 0.001, n.s. non-significant. (c) Wild type and p32^−/− MEFs were transfected with pcDNA or a p32 expression vector as indicated and subjected to Western analysis for hexokinase 2 (HK2), p32 and β-actin. (d) Wild type and p32^−/− MEFs were incubated in complete glucose-free medium for the indicated time and subjected to MTT assay. % of cell viability shown with SD as error bars. Student’s t test, *p < 0.05, ***p < 0.001, n.s. non-significant. (e) Wild type and p32^−/− MEFs were treated with 5 mM 2-deoxy-d-glucose for the indicated time and subjected to MTT assay. % of cell viability shown with SD as error bars. Student’s t test, *p < 0.05, ***p < 0.001, n.s. non-significant.