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. 2020 Jun 24;10:998. doi: 10.3389/fonc.2020.00998

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Copyright © 2020 Zhang, Cheng, Xia, Huang, He and Liao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LncRNA-NEAT1 sustains the growth of SNU-182 cells under hypoxic conditions. (A) The expression of lncRNA-NEAT1 was modulated by transfection with the pcDNA3.1-NEAT1 vector or siRNA-NEAT1. After transfection for 24 h, cells were cultured under hypoxic or normoxic conditions. Cell viability was detected using CCK-8 assay and used to estimate proliferation. (B,C) The roles of lncRNA-NEAT1 on apoptosis (Panel b) and the cell cycle (Panel c) in SNU-182 cells under hypoxic and normoxic conditions were assessed by flow cytometry. *P < 0.05 compared with transfection negative control. ^#P < 0.05 compared with pcDNA3.1-NEAT1.