TABLE 3.
Difference in HBV‐DNA Viral Loads (log10 IU/mL): Cobas HBV Versus Cobas HBV+RT and Cobas HBV Versus Aptima HBV, Cobas HPS Versus Cobas HBV and Aptima HBV Versus HBV+RT, and Aptima HBV Versus Cobas HPS
| Week | Test Comparison | Number of Samples | Mean ± SD (log10 IU/mL) | Adjusted* P Value for Difference |
|---|---|---|---|---|
| Baseline | cobas HBV+RT vs. cobas HBV | 49 | 0.12 ± 0.15 | 0.0000 |
| Aptima HBV vs. cobas HBV | 49 | −0.08 ± 0.26 | 1.0000 | |
| cobas HBV vs. cobas HPS | 49 | 0.12 ± 0.24 | 0.0205 | |
| Aptima HBV vs. cobas HBV+RT | 49 | −0.20 ± 0.29 | 0.0005 | |
| Aptima HBV vs. cobas HPS | 49 | 0.04 ± 0.29 | 1.0000 | |
| 12 | cobas HBV+RT vs. cobas HBV | 49 | 0.93 ± 0.53 | 0.0000 |
| Aptima HBV vs. cobas HBV | 49 | 0.65 ± 0.4 | 0.0000 | |
| cobas HBV vs. cobas HPS | 49 | −0.17 ± 0.36 | 0.0519 | |
| Aptima HBV vs. cobas HBV+RT | 49 | −0.29 ± 0.53 | 0.0151 | |
| Aptima HBV vs. cobas HPS | 49 | 0.48 ± 0.38 | 0.0000 | |
| 24 | cobas HBV+RT vs. cobas HBV | 50 | 1.16 ± 0.64 | 0.0000 |
| Aptima HBV vs. cobas HBV | 50 | 0.89 ± 0.43 | 0.0000 | |
| cobas HBV vs. cobas HPS | 50 | −0.22 ± 0.36 | 0.0027 | |
| Aptima HBV vs. cobas HBV+RT | 50 | −0.27 ± 0.55 | 0.0426 | |
| Aptima HBV vs. cobas HPS | 50 | 0.67 ± 0.39 | 0.0000 | |
| 48 | cobas HBV+RT vs. cobas HBV | 48 | 1.16 ± 0.72 | 0.0000 |
| Aptima HBV vs. cobas HBV | 48 | 0.96 ± 0.38 | 0.0000 | |
| cobas HBV vs. cobas HPS | 48 | −0.28 ± 0.39 | 0.0003 | |
| Aptima HBV vs. cobas HBV+RT | 48 | −0.20 ± 0.62 | 0.9520 | |
| Aptima HBV vs. cobas HPS | 48 | 0.68 ± 0.4 | 0.0000 | |
| 72 | cobas HBV+RT vs. cobas HBV | 25 | 0.97 ± 0.68 | 0.0000 |
| Aptima HBV vs. cobas HBV | 25 | 1.06 ± 0.65 | 0.0000 | |
| cobas HBV vs. cobas HPS | 25 | −0.26 ± 0.59 | 1.0000 | |
| Aptima HBV vs. cobas HBV+RT | 25 | 0.09 ± 0.63 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 25 | 0.80 ± 0.55 | 0.0000 | |
| 84 | cobas HBV+RT vs. cobas HBV | 15 | 0.80 ± 0.68 | 0.0144 |
| Aptima HBV vs. cobas HBV | 15 | 1.11 ± 0.45 | 0.0000 | |
| cobas HBV vs. cobas HPS | 15 | −0.37 ± 0.59 | 0.9959 | |
| Aptima HBV vs. cobas HBV+RT | 15 | 0.31 ± 0.73 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 15 | 0.74 ± 0.43 | 0.0004 | |
| 96 | cobas HBV+RT vs. cobas HBV | 20 | 0.85 ± 0.75 | 0.0026 |
| Aptima HBV vs. cobas HBV | 20 | 0.89 ± 0.64 | 0.0002 | |
| cobas HBV vs. cobas HPS | 20 | −0.37 ± 0.63 | 0.5458 | |
| Aptima HBV vs. cobas HBV+RT | 20 | 0.04 ± 0.55 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 20 | 0.52 ± 0.65 | 0.0771 |
All 256 valid observations with complete paired results are included. Target not detected observations are imputed with a zero value. Values below the LLOQ are imputed as 0.5 × LLOQ = 5 IU/mL for cobas HBV, cobas HBV+RT and Aptima HBV, and imputed as 0.5 × LLOQ = 14.5 IU/mL for the cobas HPS assay. Only complete data within each week across tests are included.
*
Adjusted for multiplicity using the Bonferroni method.