TABLE 4.
Difference in HBV‐DNA Viral Loads (log10 IU/mL): Cobas HBV Versus Cobas HBV+RT and Cobas HBV Versus Aptima HBV, Cobas HPS Versus Cobas HBV and Aptima HBV Versus HBV+RT, and Aptima HBV Versus Cobas HPS in 8 Patients With Samples Available at All Seven Time Points Across All HBV Tests
| Week | Test Comparison | Number of Samples | Mean ± SD (log10 IU/mL) | Adjusted* P Value for Difference |
|---|---|---|---|---|
| Baseline | cobas HBV+RT vs. cobas HBV | 8 | 0.15 ± 0.11 | 0.1928 |
| Aptima HBV vs. cobas HBV | 8 | 0.06 ± 0.22 | 1.0000 | |
| cobas HBV vs. cobas HPS | 8 | 0.02 ± 0.16 | 1.0000 | |
| Aptima HBV vs. cobas HBV+RT | 8 | −0.09 ± 0.21 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 8 | 0.09 ± 0.12 | 1.0000 | |
| 12 | cobas HBV+RT vs. cobas HBV | 8 | 1.17 ± 0.44 | 0.0050 |
| Aptima HBV vs. cobas HBV | 8 | 0.94 ± 0.33 | 0.0033 | |
| cobas HBV vs. cobas HPS | 8 | −0.36 ± 0.19 | 0.0413 | |
| Aptima HBV vs. cobas HBV+RT | 8 | −0.24 ± 0.51 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 8 | 0.58 ± 0.26 | 0.0153 | |
| 24 | cobas HBV+RT vs. cobas HBV | 8 | 1.41 ± 0.46 | 0.0019 |
| Aptima HBV vs. cobas HBV | 8 | 1.19 ± 0.3 | 0.0003 | |
| cobas HBV vs. cobas HPS | 8 | −0.37 ± 0.25 | 0.1444 | |
| Aptima HBV vs. cobas HBV+RT | 8 | −0.21 ± 0.58 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 8 | 0.82 ± 0.19 | 0.0002 | |
| 48 | cobas HBV+RT vs. cobas HBV | 8 | 1.42 ± 0.57 | 0.0075 |
| Aptima HBV vs. cobas HBV | 8 | 1.2 ± 0.31 | 0.0004 | |
| cobas HBV vs. cobas HPS | 8 | −0.38 ± 0.25 | 0.1329 | |
| Aptima HBV vs. cobas HBV+RT | 8 | −0.21 ± 0.65 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 8 | 0.82 ± 0.25 | 0.0011 | |
| 72 | cobas HBV+RT vs. cobas HBV | 8 | 1.23 ± 0.52 | 0.0099 |
| Aptima HBV vs. cobas HBV | 8 | 1.08 ± 0.63 | 0.0638 | |
| cobas HBV vs. cobas HPS | 8 | −0.1 ± 0.61 | 1.0000 | |
| Aptima HBV vs. cobas HBV+RT | 8 | −0.16 ± 0.75 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 8 | 0.97 ± 0.35 | 0.0038 | |
| 84 | cobas HBV+RT vs. cobas HBV | 8 | 1.17 ± 0.59 | 0.0289 |
| Aptima HBV vs. cobas HBV | 8 | 1.07 ± 0.51 | 0.0194 | |
| cobas HBV vs. cobas HPS | 8 | −0.08 ± 0.51 | 1.0000 | |
| Aptima HBV vs. cobas HBV+RT | 8 | −0.1 ± 0.68 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 8 | 0.99 ± 0.2 | 0.0001 | |
| 96 | cobas HBV+RT vs. cobas HBV | 8 | 1.14 ± 0.83 | 0.2133 |
| Aptima HBV vs. cobas HBV | 8 | 0.98 ± 0.8 | 0.3558 | |
| cobas HBV vs. cobas HPS | 8 | −0.17 ± 0.71 | 1.0000 | |
| Aptima HBV vs. cobas HBV+RT | 8 | −0.16 ± 0.67 | 1.0000 | |
| Aptima HBV vs. cobas HPS | 8 | 0.81 ± 0.53 | 0.1188 |
All 256 valid observations with complete paired results across all seven selected time points, across all assays, are included. Target not detected observations are imputed with a zero value. Values below the LLOQ are imputed as 0.5 × LLOQ = 5 IU/mL for cobas HBV, cobas HBV+RT and Aptima HBV, and imputed as 0.5 × LLOQ = 14.5 IU/mL for the cobas HPS assay. Only complete data within each week across tests are included.
Adjusted for multiplicity using the Bonferroni method.