Table 2.

Primers and Probes Used in Digital PCR

Target*	Oligo	Sequence	Position in HBV Genome
5′ precore RNA	F	GCACCAGCACCATGCAACTT	1803‐1822
	R	TCAGAAGGCAAAAACGAGAGTAACT	1941‐1965
	Probe	ACTGTTCAAGCCTCCAAGCTGTGCCTT	1859‐1885
pgRNA (5′ core)	F	TCACCTCTGCCTAATCATCTCTTG	1825‐1848
	R	TCAGAAGGCAAAAACGAGAGTAACT	1941‐1965
	Probe	ACTGTTCAAGCCTCCAAGCTGTGCCTT	1859‐1885
Core	F	GGTCCCCTAGAAGAAGAACTCCCT	2361‐2384
	R	CATTGAGATTCCCGAGATTGAGAT	2425‐2448
	Probe	CGCCGCGTCGCA	2409‐2420
S	F	TCCTCCAAYTTGTCCTGGTYATC	349‐371
	R	AGATGAGGCATAGCAGCAGGAT	410‐431
	Probe	CCGCAGACACATCC	375‐388
X	F	CGTCTGTGCCTTCTCATCTG	1550‐1569
	R	GCGTTCACGGTGGTCTCCA	1609‐1627
	Probe	CACTTCGCTTCACCTC	1582‐1597
3′ precore RNA	F	GGAGGCTGTAGGCATAAATTGGTC	1776‐1799
	R	TTCTTTATAAGGGTCAATGTCCATG	1900‐1924
	Probe	CCTCCAAGCTGTGCC	1869‐1883
cccDNA	F	CCGTGTGCACTTCGCTTCA	1575‐1593
	R	GCACAGCTTGGAGGCTTGA	1864‐1882
	Probe	CATGGAGACCACCGTGAACGCCC	1607‐1629
Beta‐globin DNA	F	GCTCATGGCAAGAAAGTGCTC
	R	GCAAAGGTGCCCTTGAGGT
	Probe	AGTGATGGCCTGGCTCACCTGGAC

^*Minor groove‐binding probes were used in core RNA, S RNA, X RNA, and 3′ precore RNA assays.

Abbreviations: F, forward; R, reverse.