Table 1.
Unsaturated phospholipid fatty acids profiles in groups of keratinocytes: I. control; II. cultured with CBD (4uM) for 24h; III. cultured with CBD (4uM) for 48h; VI. irradiated with UVB [60 mJ/cm2]; V. irradiated with UVB [60 mJ/cm2] and cultured with CBD (4uM) for 24h after irradiation; VI. irradiated with UVB [60 mJ/cm2] and cultured with CBD for 24h befor irradiation and 24h after irradiation; VII. exposed to H2O2 [200μM]; VIII. exposed to H2O2 [200μM] and cultured with CBD (4uM) for 24h after exposer to H2O2; IX. exposed to H2O2 [200μM] and cultured with CBD (4uM) for 24h before and 24h after exposer to H2O2. Mean values ± SD of five independent experiments and statistically significant differences for p < 0.05 are presented:a - differences vs. group I/group IV/group VII; b - differences vs. group II/group V/group VIII; x - differences between group IV/group VII and group I.
| Control groups |
Keratinocytes treated with UVB |
Keratinocytes treated with H2O2 |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Fatty acids μg/mg of protein |
Control (I) | CBD treatment (II) | CBD pretreatment + treatment (III) |
UVB (IV) | CBD treatment (V) | CBD pretreatment + treatment (VI) |
H2O2(VII) | CBD treatment (VIII) | CBD pretreatment + treatment (IX) |
| Oleic (C18:1n9c) | 69.2 ± 3.4 | 80.6 ± 4.0a | 77.7 ± 3.9a | 23.4 ± 1.2x | 67.7 ± 3.4ab | 77.3 ± 3.9ab | 82.6 ± 4.1x | 77.1 ± 3.8 | 70.7 ± 3.5a |
| Linoleic (C18:2n6c) | 21.6 ± 1.1 | 25.3 ± 1.3a | 22.5 ± 1.1b | 7.9 ± 0.4x | 22.4 ± 1.1a | 18.4 ± 0.9ab | 28.2 ± 1.1x | 22.1 ± 1.1a | 18.9 ± 0.9ab |
| γ-Linolenic (C18:3n6) | 7.44 ± 0.37 | 8.86 ± 0.44a | 7.16 ± 0.36b | 2.70 ± 0.13x | 7.57 ± 0.38a | 6.89 ± 0.34a | 10.13 ± 0.51x | 8.40 ± 0.42a | 7.23 ± 0.36ab |
| Linolenic (C18:3n3) | 0.10 ± 0.01 | 0.12 ± 0.01 | 0.11 ± 0.01 | 0.03 ± 0.01x | 0.09 ± 0.01a | 0.11 ± 0.01ab | 0.16 ± 0.01x | 0.13 ± 0.01a | 0.11 ± 0.01a |
| Arachidonic (C20:4n6) | 8.48 ± 0.42 | 9.98 ± 0.50a | 9.15 ± 0.46 | 2.97 ± 0.15x | 8.61 ± 0.43a | 8.72 ± 0.44a | 10.22 ± 0.51x | 9.11 ± 0.46a | 8.10 ± 0.40ab |
| cis-5,8,11,14,17-Eicosapentaenoic (C20:5n3) | 4.40 ± 0.22 | 5.16 ± 0.26a | 4.44 ± 0.22b | 1.51 ± 0.08x | 4.30 ± 0.22a | 4.50 ± 0.22a | 5.33 ± 0.27x | 4.64 ± 0.23a | 4.23 ± 0.21a |
| cis-4,7,10,13,16,19-Docosahexaenoic (C22:6n3) | 2.60 ± 0.13 | 3.23 ± 0.16a | 3.05 ± 0.15a | 0.91 ± 0.05x | 2.67 ± 0.13a | 3.11 ± 0.16ab | 3.20 ± 0.16x | 3.00 ± 0.15 | 2.84 ± 0.14a |