Fig. 7.

Iron-exposed monocytes reduce bortezomib sensitivity of myeloma PCs. A) Experimental paradigm of primary human monocytes (Mϕ) and U266 cells cocultures. Representative dot plots (B) and quantification (C) of apoptotic cells after BTZ treatment in U266 cells obtained from coculture with Mϕ (U266^Mϕ) or from coculture with Mϕ/FAC (U266^Mϕ/FAC). Data are shown as mean percentage ±SEM; ***p-value < 0.001 and **p-value < 0.01 versus controls or between groups. D) Quantification of mitochondrial mass analysis using MitoTracker probe in U266^Mϕ and U266^Mϕ/FAC cells; data are shown as mean of MFI ±SEM; E) TFAM mRNA levels U266^Mϕ and U266^Mϕ/FAC cells; data are expressed as mean of FC over control ±SEM; *p-value < 0.05 and **p-value < 0.01 versus control.