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. 2020 May 13;39(13):e103695. doi: 10.15252/embj.2019103695

Figure EV3. Dephosphorylation of FoxO3 by PP2A‐B56.

Figure EV3

  1. Validation of FoxO3 α‐pS253 antibody. The indicated YFP‐FoxO3 variants were immunopurified and subjected to Western blotting with the indicated antibodies. CIP; calf intestinal phosphatase, 2A; FoxO3 M448A I451A unable to bind PP2A‐B56.
  2. Dephosphorylation by the PP2A‐B56α holoenzyme complex of GST‐Cdc20 49–78 substrates with LxxIxE motifs of different binding affinities for B56 as indicated. The mean and standard deviation of 3 independent experiments are shown.

Source data are available online for this figure.