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Binding affinities for wt and its variants based on FA experiments
| KD (μM) | |
|---|---|
| Wild-type | 5.1 ± 0.4 |
| R1281A | 17.6 ± 1.0 |
| S1283A | 5.4 ± 0.2 |
| S1284A | 8.2 ± 0.1 |
| R1285A | 12.0 ± 0.3 |
| E1324A | 1.4 ± 0.1 |
| K1354E | 16.3 ± 1.7 |
| K1360A | 12.4 ± 0.5 |
| K1434A | 24.0 ± 2.7 |
| R1281A/S1283A | 35.5 ± 1.9 |
| R1281A/S1284A | 11.4 ± 1.0 |
| R1281A/R1285A | 11.6 ± 0.9 |
| R1281A/S1283A/R1285A | 16.5 ± 0.5 |
| R1281E/S1284A/R1285E | 48.3 ± 4.7 |
| Spt6-N-SH2 | 313.2 ± 12.5 |
