Figure S2.

Plk4-NG Oscillations in Individual Embryos, Related to Figures 1 and 5

(A) Graphs show the Mean ± SD centriolar fluorescence intensity of Plk4-NG (two copies of a transgene expressed from its own promoter in a Plk4 null mutant background) during nuclear cycles 11-13 in 5 different embryos imaged on a spinning-disk confocal system. n = 26 centrioles (mean) tracked starting from cycle 11 per embryo. See STAR Methods for full details of image acquisition and data analysis.

(B) Same as in (A), but showing the Plk4-NG oscillation in 5 embryos arrested in interphase by the injection of dsRNAs against Cyclin A, B and B3.

See Figure 5A for further details on sample numbers and experimental protocol.