Table 1.
Antibodies used in the experiment.
| Antibody name | Product code | Source | Host | Dilution |
|---|---|---|---|---|
| HK2 | #2867 | Cell signaling technology | Rabbit | 1 : 1000 |
| PFKP | #5412 | Cell signaling technology | Rabbit | 1 : 1000 |
| PKM2 | #4053 | Cell signaling technology | Rabbit | 1 : 1000 |
| PKM1/2 | #3186 | Cell signaling technology | Rabbit | 1 : 1000 |
| PDHX | #3205 | Cell signaling technology | Rabbit | 1 : 1000 |
| AKT | #4691 | Cell signaling technology | Rabbit | 1 : 500 |
| p-Akt (Thr308) | #2965 | Cell signaling technology | Rabbit | 1 : 1000 |
| p-Akt (Ser473) | 33281 M | Bioss | Mouse | 1 : 500 |
| mTOR | #2983 | Cell signaling technology | Rabbit | 1 : 1000 |
| p-mTOR (Ser2448) | #5536 | Cell signaling technology | Rabbit | 1 : 1000 |
| HIF-1α | #3716 | Cell signaling technology | Rabbit | 1 : 1000 |
| HK1 | ab65069 | Abcom | Rabbit | 1 : 500 |
| GLUT1 | ab652 | Abcom | Rabbit | 1 : 500 |
| LDHA | ab125683 | Abcom | Rabbit | 1 : 1000 |
| β-Actin | ab 179467 | Abcom | Rabbit | 1 : 1000 |
| Caspase 3/p17/19 | 19677-10-AP | Proteintech | Rabbit | 1 : 1000 |
| BAX | 50599-2-lg | Proteintech | Rabbit | 1 : 4000 |
| Bcl 2 | 26593-1-AP | Proteintech | Rabbit | 1 : 1000 |
| Goat anti-rabbit IgG | ZB-2301 | Zsbio | Goat | 1 : 5000 |
| Goat anti-mouse IgG | ZB-2305 | Zsbio | Goat | 1 : 5000 |