Table 2.
Primer sequences used for RT-PCR.
| Gene | Primer | Sequence |
|---|---|---|
| HK1 | Forward | 5′-GCTCTCCGATGAAACTCTCATAG-3′ |
| Reverse | 5′-GGACCTTACGAATGTTGGCAA-3′ | |
|
| ||
| HK2 | Forward | 5′-CCCGTGCCCACAATGAGAC-3′ |
| Reverse | 5′-CCCGTGCCCACAATGAGAC-3′ | |
|
| ||
| PFKP | Forward | 5′-GCATGGGTATCTACGTGGGG-3′ |
| Reverse | 5′-CTCTGCGATGTTTGAGCCTC-3′ | |
|
| ||
| PKM1/2 | Forward | 5′-ATGTCGAAGCCCCATAGTGAA-3′ |
| Reverse | 5′-TGGGTGGTGAATCAATGTCCA-3′ | |
|
| ||
| PDHX | Forward | 5′-TTGGGAGGTTCCGACCTGT-3′ |
| Reverse | 5′-CAACCACTCGACTGTCACTTG-3′ | |
|
| ||
| GLUT1 | Forward | 5′-TCATCGTCGCTGAACTCTTCAG-3′ |
| Reverse | 5′-TCACACTTGGGAATCACCCCC-3′ | |
|
| ||
| LDHA | Forward | 5′-ATGGCAACTCTAAAGGATCAGC-3′ |
| Reverse | 5′-CCAACCCCAACAACTGTAATCT-3′ | |