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. 2019 Oct 3;105(7):1802–1812. doi: 10.3324/haematol.2019.216010

Figure 2.

Figure 2

Proximity ligation assay of GATA1 complexes in E12.5 fetal liver tissue. (A) Proximity ligation assay (PLA) for GATA1/FOG1 and GATA1/LDB1 interactions was performed on sections of mouse fetal liver (FL) tissue at embryonic day 12.5 (E12.5), together with GATA1, FOG1 or LDB1 single-primary antibody negative controls. PLA protein interactions were visualized in red, and DAPI staining in blue was used to visualize the nucleus (scale bars, 50 μm in 20x and 40x). For each inter-action zoom-in pictures corresponding to the white square area are also shown (scale bars, 20 μm in 63x). Z-stack images of each protein combination and fluorescent channels were projected by Maxi-Projection algorithm. (B) PLA for GATA1/LMO2 was performed as in Panel A using LMO2 antibodies replacing FOG1 antibodies. Scale bars represent 50 μm apart fromscale bars in zoom in picures bars which represent 10 μm.