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. 2020 May 6;11(7):1801–1816. doi: 10.1111/1759-7714.13450

Figure 4.

Figure 4

miR‐449b‐5p suppresses the expression of HSPA1A by targeting the mRNA 3′‐UTR of HSPA1A. (a and b) The BRCA cell lines MDA‐MB‐231 (a) and MCF‐7 (b) were transfected with miR‐449b‐5p or control 12 hours before the irradiation treatment. Then, 36 hours after the irradiation, total RNA and protein were extracted. The mRNA and protein levels of HSPA1A were detected by real‐time PCR and western blot analysis, respectively. (c and d) The BRCA cell lines MDA‐MB‐231 (c) and MCF‐7 (d) were transfected with the miR‐449b‐5p inhibitor or control 12 hours before the irradiation treatment. A total of 36 hours after the irradiation, total RNA and protein were extracted. The mRNA and protein levels of HSPA1A were detected by real‐time PCR and western blot analysis, respectively. Data are shown as mean ± SD of three independent experiments. Statistical significant differences are indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001; Student's t‐test.