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. 2020 May 6;11(7):1801–1816. doi: 10.1111/1759-7714.13450

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© 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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HOTAIR enhances the proliferation of irradiated‐BRCA cells through HSPA1A in vitro. The grouping of the test was as follows: Ctrl, IR (10 or 6 Gy), IR (10 or 6 Gy) + HOTAIR, IR (10 or 6 Gy) + HOTAIR+miR‐449b‐5p and IR (10 or 6 Gy) + HOTAIR+si‐HSPA1A. (a) The expression of HSPA1A at mRNA and protein levels in MDA‐MB‐231 cells from each group was assessed by real‐time PCR and western blot analysis, respectively. (b) The cell viability of MDA‐MB‐231 cells from each group was determined by CCK‐8 assay. () Ctrl, () IR 10 Gy, () IR 10 Gy + HOTAIR, () IR 10 Gy + HOTAIR+miR‐449b‐5p, () IR 10 Gy + HOTAIR + si‐HSPA1A (c and d) The proliferative ability of MDA‐MB‐231 cells from each group was validated by colony formation assay (c) and EdU incorporation assay (d, scale bar, 30 μM), respectively. The colony formation efficiency and percentage of EdU positive cells were calculated. Data are shown as mean ± SD of three independent experiments. Statistical significant differences are indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, no significance; Student's t‐test.

Inline graphic — HOTAIR enhances the proliferation of irradiated‐BRCA cells through HSPA1A in vitro. The grouping of the test was as follows: Ctrl, IR (10 or 6 Gy), IR (10 or 6 Gy) + HOTAIR, IR (10 or 6 Gy) + HOTAIR+miR‐449b‐5p and IR (10 or 6 Gy) + HOTAIR+si‐HSPA1A. (a) The expression of HSPA1A at mRNA and protein levels in MDA‐MB‐231 cells from each group was assessed by real‐time PCR and western blot analysis, respectively. (b) The cell viability of MDA‐MB‐231 cells from each group was determined by CCK‐8 assay. () Ctrl, () IR 10 Gy, () IR 10 Gy + HOTAIR, () IR 10 Gy + HOTAIR+miR‐449b‐5p, () IR 10 Gy + HOTAIR + si‐HSPA1A (c and d) The proliferative ability of MDA‐MB‐231 cells from each group was validated by colony formation assay (c) and EdU incorporation assay (d, scale bar, 30 μM), respectively. The colony formation efficiency and percentage of EdU positive cells were calculated. Data are shown as mean ± SD of three independent experiments. Statistical significant differences are indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, no significance; Student's t‐test.