Fig. 3.

Lanthipeptide biosynthetic logic and mining strategy. A) Exemplified enzymatic mechanism of lanthipeptide biosynthesis. LP, leader peptide. Xn, peptide with n residues. B) Nisin biosynthetic gene cluster from Lactococcus lactis. C) Lanthipeptide mining strategy. (i) Tailoring enzyme-based mining mainly focuses on identifying the hallmark of lanthipeptide biosynthesis, such as dehydratase and cyclase. (ii) MS/MS matching connects genotypes and chemotypes. The MS data of predicated mature peptides was aligned with the experimentally obtained MS data to guide target isolation.