Fig. 7.

Sequence alignment of AT domains. Signature motifs including YASH for methylmalonyl-CoA, HAFH for malonyl-CoA, TAGH for ethylmalonyl-CoA, and less conserved motifs for rarer extender units as well as the diagnostic Q versus I/L motif indicating methylmalonyl versus malonyl-CoA are highlighted in cyan. The active site histidine/serine dyad is highlighted in yellow. Mutations introduced by Schulz and coworkers in EryAT6 are indicated in magenta [76,77,115], those introduced by Williams and coworkers [72] are indicated in green, and those explored by both the Williams and Schulz groups are indicated in grey. The alignment was performed with MUSCLE.