Figure 2.

AAV Transduction in Cerebral Organoids

(A,B,D,E) Representative images of two organoids transduced with rAAV5-GFP- (A,B) and two organoids transduced with rAAV9-GFP- (D,E) show expression of GFP transgene with both serotypes. GFP was stained with αGFP antibody, and nuclei were dyed with DAPI. The images are a maximum projection of a tile scan of z stacks acquired by confocal imaging. (E) Arrowhead shows a suture fragment that is added during organoid formation for improved morphology (see Materials and Methods). (C) and (F) are zoomed-in regions of (B) and (E), respectively. (A,B,D,E) scale bar is 500 μm, (C,F) scale bar is 250 μm.