Figure 4.

Comparing rAAV5 and rAAV9 Transduction Based on vDNA and SEAP Transgene Activity in 2D Neuronal Cultures

(A) rAAV5-SEAP transduction of 2D iPSC-derived neuronal cultures led to higher levels of vDNA compared with rAAV9-SEAP as measured by qPCR. Only at a dose of 10¹² genome copies (gc)/well was the increase significant (n = 3 wells; two-way ANOVA, Sidak’s multiple comparison, ∗∗∗∗p < 0.0005; mean with 95% CI). (B) rAAV5-SEAP transduction led to a significantly increased activity of transgenic SEAP compared with rAAV9-SEAP in all three doses tested, as measured by a luminescence assay (n = 3 wells; two-way ANOVA, Sidak’s multiple comparison, ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005; mean with 95% CI). The difference between the serotypes was the most pronounced at lower doses.