Figure 5.

Introduction of an Additional 2′-Fluoro in the Guide Strand Seed Does Not Impact the Thermal Melting Temperatures of siRNA Guide Strands and Their RNA Targets within the Seed Region

(A) Schematic representations of guide strand chemical modification patterns used; symbols next to each schematic are used in graphs shown in (B). Legend shows corresponding chemical structures for 2′-ribose chemistries. (B) Thermal melt curves for siRNA guide strands (positions 1–8) and their targets within the seed region (i.e., guide strand positions 2–8). Target name and start site of target sequence are indicated in graphs; Tm values are displayed in graph legends.